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BS-C-1
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HE Hopps
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Cercopithecus aethiops
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epithelial
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Organ: kidney
Disease: normal
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keratin
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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transfection host (Roche FuGENE® Transfection Reagents)
detection of viruses [21447]
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Chromosome Frequency Distribution 100 Cells: 2n = 60One large submetacentric marker chromosome. Most of the cells contained a chromosome with a secondary constriction and many of the cells contained dicentric chromosomes.
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
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Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Two to three times weekly
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Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
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1820: Hopps, H. E., et al. Biologic characteristics of the continuous kidney cell line derived from the African green monkey. J. Immunol. 91: 416-424, 1963. PubMed: 14071033
21447: American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.
32372: Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256
32501: Jelachich ML, Lipton HL. Theiler's murine encephalomyelitis virus kills restrictive but not permissive cells by apoptosis. J. Virol. 70: 6856-6861, 1996. PubMed: 8794327
58406: Schmidt NJ, et al. The sensitivity of grivet monkey kidney cell line BS-C-1 for propagation and isolation of certain human viruses. Am. J. Public Health 54: 1522-1530, 1964.
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