產品[
CRL-2190 HK-2 人腎近曲小管上皮細胞
]資料
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產品名稱:
CRL-2190 HK-2 人腎近曲小管上皮細胞
產品型號:
CRL-2190
產品廠商:
美國標準生物品收藏中心（ATCC）
產品文檔:
無相關文檔
簡單介紹
CRL-2190 HK-2 人腎近曲小管上皮細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！
CRL-2190 HK-2 人腎近曲小管上皮細胞
的詳細介紹
CRL-2190 HK-2 人腎近曲小管上皮細胞
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CRL-2190™ |
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$272.00 |
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HK-2 |
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RA Zager |
| Biosafety Level: |
2 [Cells Contain Papilloma viral DNA sequences ] |
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frozen |
| Medium & Serum: |
See Propagation |
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adherent |
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Homo sapiens (human) |
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epithelial
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Organ: kidney, cortex
Tissue: proximal tubule
Cell Type: human papillomavirus 16 (HPV-16) transformed |
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alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin |
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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epidermal growth factor (EGF), expressed |
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Amelogenin: X,Y
CSF1PO: 13
D13S317: 9
D16S539: 11,12
D5S818: 12
D7S820: 10,11
THO1: 9
TPOX: 8,9
vWA: 17,18 |
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***** |
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male |
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ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
- 0.05 mg/ml BPE - provided with the K-SFM kit
- 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.
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Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
- Incubate cultures at 37°C.
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